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Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Genotyping, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genotyping - by Bioz Stars, 2026-03
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mouse tail genotype rapid identification kit  (Beyotime)
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Beyotime mouse tail genotype rapid identification kit
Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Mouse Tail Genotype Rapid Identification Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genotypic rapid identification kit #d7283s  (Beyotime)
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Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Genotypic Rapid Identification Kit #D7283s, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genotypic rapid identification kit  (Beyotime)
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Beyotime genotypic rapid identification kit
Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
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genotypic rapid identification kit - by Bioz Stars, 2026-03
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Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Rapid Genotyping #4359187, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rapid genotyping #4359187 - by Bioz Stars, 2026-03
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hpv genotyping kit hybribio rapid genoarray  (HybriBio Limited)
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HybriBio Limited hpv genotyping kit hybribio rapid genoarray
Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Hpv Genotyping Kit Hybribio Rapid Genoarray, supplied by HybriBio Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse tail genotyping rapid identification kit  (Beyotime)
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Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Mouse Tail Genotyping Rapid Identification Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hpv genotyping kit rapid hpv genotyping macroarray  (HybriBio Limited)
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Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Hpv Genotyping Kit Rapid Hpv Genotyping Macroarray, supplied by HybriBio Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rapid genotyping assay – cyp2c9 & vkorci/  (Gentris Corporation)
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Gentris Corporation rapid genotyping assay – cyp2c9 & vkorci/
Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) <t>Genotyping</t> of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.
Rapid Genotyping Assay – Cyp2c9 & Vkorci/, supplied by Gentris Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) Genotyping of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.

Journal: Neural Regeneration Research

Article Title: Selective deletion of zinc transporter 3 in amacrine cells promotes retinal ganglion cell survival and optic nerve regeneration after injury

doi: 10.4103/1673-5374.373660

Figure Lengend Snippet: Specific knockout of ZnT3 in ACs and RGCs. (A) Schematic illustration of the ZnT3 ( Slc30a3 ) knockout strategy by CRISPR/Cas9 technology. (B) Schematic of the retinal structure (left); VGLUT2-Cre mice selectively express Cre in RGCs (green), whereas VGAT-Cre mice selectively express Cre in amacrine (red) and horizontal cells (black). Synaptic connections between RGCs and ACs are shown on the right; Zn 2+ is encapsulated in the synaptic vesicles in ACs, ZnT3 transports the vesicles to the synaptic cleft, and Zn 2+ released into RGCs. (C) Genotyping of VGAT-Cre (mut/wt), Slc30a3 fl/fl , and Slc30a3 fl/fl VGAT-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGAT-Cre mice. VGAT-Cre: Mutant = 200 bp, wt = 323 bp; Slc30a3 : fl = 304 bp, wt = 202 bp. (D) Genotyping of VGLUT2-Cre (mut/wt), Slc30a3 fl/fl and Slc30a3 fl/fl VGLUT2-Cre mice ( n = 4). The white boxes indicate the genotyping result of Slc30a3 fl/fl VGLUT2-Cre mice. VGUT2-Cre: Mutant = 124 bp, wt = 245 bp; Slc30a3: fl = 304 bp, wt = 202 bp. AC: Amacrine cell; CRISPR/Cas9: clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9; RGCs: retinal ganglion cells; wt or wt/wt: C57BL/6J wild-type mice; VGAT: vesicular γ-aminobutyric acid transporter; VGLUT2: vesicular-glutamate transporter 2; ZnT3: zinc transporter 3.

Article Snippet: Genotyping was performed by polymerase chain reaction using DNA polymerase (Vazyme, Nanjing, China, Cat# P222).

Techniques: Knock-Out, CRISPR, Mutagenesis